Course Title: Perform tissue and cell culture techniques

Part B: Course Detail

Teaching Period: Term1 2017

Course Code: BIOL5234C

Course Title: Perform tissue and cell culture techniques

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5363 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amber Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Muriel Aldunate  

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites



MSL973007 Perform microscopic examination

MSL973004 Perform aseptic techniques

Course Description

This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment set-up, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975013 Perform tissue and cell culture techniques


1 Interpret and schedule production requirements

Performance Criteria:

1.1 Review client request and confirm quantity and nature of cells, tissue or products 1.2 Select appropriate media, materials, equipment and methods 1.3 Plan parallel work sequences to optimise production 1.4 Maintain a chain of custody, traceable to the worker, for all cells and tissues


2 Work safely according to the legal and regulatory framework

Performance Criteria:

2.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures 2.2 Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment 2.3 Select, fit and use personal protective equipment (PPE) 2.4 Address hazards and incidents as they arise 2.5 Ensure the safe disposal of biohazardous materials and other laboratory waste


3 Assemble and maintain tissue culture equipment

Performance Criteria:

3.1 Assemble, sterilise or decontaminate equipment according to workplace procedures 3.2 Perform pre-use and safety checks in accordance with relevant workplace and operating procedures 3.3 Identify faulty or unsafe components and equipment and report to appropriate personnel 3.4 Decontaminate area and equipment after use


4 Prepare and test cell and tissue culture media

Performance Criteria:

4.1 Confirm media specifications and processes/methods 4.2 Prepare culture media to suit client request 4.3 Sterilise culture media and check for sterility 4.4 Perform quality control checks to ensure that culture media is fit for purpose 4.5 Store culture media in accordance with specifications


5 Obtain, monitor and maintain tissue and cell lines

Performance Criteria:

5.1 Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture 5.2 Select specified culture media and add any necessary growth agents or nutrients 5.3 Incubate cells/tissue in specified conditions 5.4 Inoculate the media with the specified amount of sample 5.5 Monitor growth of tissue and cell lines and products 5.6 Detect contamination and troubleshoot materials, equipment and techniques 5.7 Passage samples by subculturing to preserve or grow the line 5.8 Harvest cells or cell products to optimise yields


6 Preserve cells and tissues

Performance Criteria:

6.1 Select the appropriate preservation method 6.2 Preserve the cell lines or tissue in accordance with the method 6.3 Check preserved cell lines regularly to ensure viability is maintained


7 Maintain records

Performance Criteria:

s 7.1 Maintain records of batches of media and test data 7.2 Maintain records of active and stored tissue and cell lines7.3 Ensure records are retrievable, legible and accurate 7.4 Ensure records conform to the information management, records, quality system and legal requirements

Learning Outcomes

Details of Learning Activities

  • Teacher directed delivery of lessons
  • Class discussion
  • Worksheets 
  • Laboratory experiments 
  • Record keeping of experiments 
  • Research activities
  • group work

Teaching Schedule

Wk. no.

Week Beginning 





Mon 6th Feb

TOPIC 1 - Intro to biohazard cabinets, aseptic technique and tissue culture


TOPIC 2 - Intro to plant tissue culture and plant physiology


PRAC 1 : Introduction to the Biohazard hood, aseptic technique and tissue culture


PRAC 2: Media preparation


Log book


Mon 13th Feb

TOPIC 3 – Plant growth

PRAC 3: Seed (Lepidium sativum) propagation


PRAC 4: Surface sterilisation preparation


Log book


Mon 20th Feb

TOPIC 4 – Plant reproduction

PRAC 5: Lepidium sativum seedling transfer


PRAC 6: Surface sterilisation and explant of African Violet leaves


Log book



Assessment 1 provided:

Prac report

(PRAC 6, 7 & 8)

DUE: Week 9



Mon 27th Feb

TOPIC 5 - Plant tissue culture environment

PRAC 6: Surface sterilisation and explant of African Violet leaves


Log book




Mon 6th Mar


TOPIC 6 – Media and growth regulators

PRAC 7: Effect of surface sterilisation and explant of Petunia leaves & Collect results from PRAC 6


Log book


Mon 13th Mar

TOPIC 7 – Micro propagation 1 &2

PRAC 8: Collect results from PRAC  7 and compare results from PRAC 6&7


Discussion of PRAC results and Assessment 1


Log book




Mon 20th Mar

TOPIC 9 – Contamination

PRAC 9: Cauliflower explant 

Log book



Mon 27th Mar

TOPIC 10 – GMO’s


PRAC 10: Carrot callus culture


Log book


Mon 3rd Apr

Assessment 2 preparation-


By end of class

PRAC 11: Succulent explant




Assessment 2 provided:GMO group presentation


DUE: Week 13 (30%)


Mon 10th Apr






Mon 17th Apr






Mon 24th Apr





Mon 1st May


PRAC 12: Collect PRAC 11 results and take plants home

collect PRAC 10 results (3 weeks post explant)

Log book


Mon 8th May




DUE (30%)


Mon 15th May


Log book check and feedback

ASSESSMENT 3: Log Book – Competency based assessment (C/NYC) (Observational and not keeping skills)
DUE Date: Week 14



Mon 22nd May


Practical exam (Explant)

ASSESSMENT 4: Practical Exam – Competency based assessment (C/NYC) (Technical skills)  


Mon 29th May



ASSESSMENT 5: End of year quiz


Semester 2 course outline will be posted to blackboard at a later date


Learning Resources

Prescribed Texts


Other Resources

Overview of Assessment

Practical reports

laboratory technical skills tests

written tests

research assignment

group presentation

laboratory logbook

Assessment Tasks

Semester 1

  • Assessment 1: Prac report. 30% Due week 9
  • Assessment 2: GMO group presentation 30% Due Week 13
  • Assessment 3: Log book (S/NYS) Due week 14
  • Assessment 4: Skills test (S/NYS) week 15
  • Assessment 5: Written test 40% week 16

Semester 2

  • Assessment 1: History of tissue culture research assignment 15% DUE Date: Week 4
  • Assessment 2: Practical report including peer review and edit. Peer review and edit (C/NYC) Report 20% DUE Date: Week 10 & 11
  • Assessment 3: Mid semester exam 25%
  • Assessment 4: Log book (15%) Due week 14
  • Assessment 5: End of Semester Exam 25%
  • Practical Exam (S/NYS)

Assessment Matrix

Other Information

Assessment Information
This course is graded in accordance with competency-based assessment, but which also utilise graded assessment

CHD Competent with High Distinction (80 – 100%)

CDI Competent with Distinction (70 – 79%)

CC Competent with Credit (60 – 69%)

CAG Competency Achieved – Graded (50 – 59%)

NYC Not Yet Competent (0 – 49%)

DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).     • Late work that is submitted without an application for an extension (see below) will not be corrected.     • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.     • For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination   A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher   Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher. 


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