Course Title: Cell and Tissue Culture

Part A: Course Overview

Course Title: Cell and Tissue Culture

Credit Points: 12.00

Terms

Course Code

Campus

Career

School

Learning Mode

Teaching Period(s)

BIOL2264

City Campus

Undergraduate

135H Applied Sciences

Face-to-Face

 Sem 1 2016

BIOL2264

City Campus

Undergraduate

171H School of Science

Face-to-Face

 Sem 1 2017,
Sem 1 2018,
Sem 1 2019,
Sem 1 2020,
Sem 1 2021,
Sem 1 2022,
Sem 1 2023,
Sem 1 2024

Course Coordinator: Dr. Ravi Shukla

Course Coordinator Phone: +61 3 9925 2970

Course Coordinator Email: ravi.shukla@rmit.edu.au

Course Coordinator Location: 003.02.020 (City campus)

Course Coordinator Availability: Email for appointment


Pre-requisite Courses and Assumed Knowledge and Capabilities

 

Required Prior Study

You should have satisfactorily completed following course/s before you commence this course.

Alternatively, you may be able to demonstrate the required skills and knowledge before you start this course.

Contact your course coordinator if you think you may be eligible for recognition of prior learning.


Course Description

This course provides knowledge of and expertise in animal and plant tissue culture theory and practice. This course has a vocational focus and introduces you to the theory and practice of animal and plant tissue culture with their role and applications in biotechnology research.

The topics covered in this course include media preparation, sterile techniques, aseptic handling, initiation, and routine maintenance of cells in culture, common contaminants of plant and animal cell culture, and introduction to some of the applications of cell culture technology including somatic cells, vaccines and Hybridoma technology etc. 

 


Objectives/Learning Outcomes/Capability Development

This course contributes to the following Program Learning Outcomes in BP229 Bachelor of Science:

 

Understanding Science.

1.1 You will be able to articulate the methods of science and explain why current scientific knowledge is contestable and testable through further inquiry.

Scientific knowledge:

2.1. You will demonstrate a depth and breadth of knowledge and understanding of biological sciences.

Inquiry and problem solving:

3.4. You will be able to analyse and solve problems in biotechnology by collecting, accurately recording, interpreting, and drawing conclusions from scientific data.

Personal and professional responsibility.

5.2 You will work responsibly, safely, legally and ethically in an individual and team context.


On successful completion of this course, you should be able to:

  1. Describe  major components of cell and tissue culture media, e.g. minerals, growth factors, hormones, and what governs the choice of components.
  2. Demonstrate knowledge of cell lines establishment for mammalian cell culture, and some of their applications. 
  3. Identify common cell culture contaminants and understand mitigation techniques. 
  4. Perform the common cell culture techniques, e.g. Embryo culture and embryogenesis in plants, culture of animal cells.
  5. Demonstrate practical application and conceptual knowledge of cell and plant tissue culture for biotechnology research and applications. 


Overview of Learning Activities

The learning activities included in this course are:

  1. lectures and lecture materials where syllabus material will be presented and explained, and the subject will be illustrated with demonstrations and examples.
  2. completion of practical work and assignments consisting of numerical and other problems requiring an integrated understanding of the subject matter; and
  3. private study, working through the course as presented in classes and learning materials, and gaining practice at solving conceptual problems. 


Overview of Learning Resources

RMIT will provide you with resources and tools for learning in this course through myRMIT Studies Course. 

There are services available to support your learning through the University Library. The Library provides guides on academic referencing and subject specialist help as well as a range of study support services. For further information, please visit the Library page on the RMIT University website and the myRMIT student portal.


Overview of Assessment

 

Assessment Tasks

Assessment Task 1: Discipline based practical competency exercises and assessment 
Weighting 30% 
This assessment task supports CLOs 1, 2, 3, & 4

Assessment Task 2: Theory Assessment (Online Quizzes)
Weighting 20%  
This assessment task supports CLOs 1, 2, 3, 4, & 5 

Assessment Task 3: Group study and presentation  
Weighting 25%  
This assessment task supports CLOs 2 & 5 

Assessment Task 4: Project report  
Weighting 25%  
This assessment task supports CLO 5 

If you have a long-term medical condition and/or disability it may be possible to negotiate to vary aspects of the learning or assessment methods. You can contact the program coordinator or Equitable Learning Services if you would like to find out more.