Course Title: Perform biological procedures

Part B: Course Detail

Teaching Period: Term1 2024

Course Code: BIOL5246C

Course Title: Perform biological procedures

School: 535T Social Care and Health

Campus: City Campus

Program: C4397 - Certificate IV in Laboratory Techniques

Course Contact: Rauha Quazi

Course Contact Phone: +61 3 9925 4277

Course Contact Email: rauha.quazi@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Course Coordinator (teacher)
Andrea McAdam
andrea.mcadam@rmit.edu.au

Room 51.7.05

Nominal Hours: 130

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

None

Course Description

This unit of competency describes the skills and knowledge to interpret work requirements, prepare samples, and perform routine biological procedures. These procedures may involve several steps and are used to classify organisms and biologically active compounds by analysing their biological and/or chemical characteristics. This unit includes interpretation of results and troubleshooting obvious departures from standard procedures.
This unit of competency applies to technical assistants working in the biomedical, environmental, biotechnology and education industry sectors.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974021 Perform biological procedures

Element:

1. Interpret and schedule work requirements

Performance Criteria:

1.1 Review work request to identify samples, required procedures and materials/equipment/instruments involved 1.2 Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment 1.3 Plan parallel work sequences to optimise throughput of multiple sets of samples

Element:

2. Receive and prepare biological samples

Performance Criteria:

2.1 Log samples using standard operating procedures (SOPs) 2.2 Record sample description, compare with specification and note and report discrepancies 2.3 Prepare samples in accordance with testing requirements

Element:

3. Perform techniques that assist in the classification of organisms

Performance Criteria:

3.1 Identify characteristics used to classify organisms 3.2 Use dichotomous keys to identify organisms

Element:

4. Perform biological procedures

Performance Criteria:

4.1 Set up and use equipment and reagents in accordance with the method 4.2 Perform techniques in accordance with the standard operating procedures

Element:

5. Maintain a safe work environment

Performance Criteria:

5.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel 5.2 Minimise the generation of waste 5.3 Safely dispose of biohazardous wastes 5.4 Clean, care for and store equipment and reagents as required

Element:

6. Maintain laboratory records

Performance Criteria:

6.1 Record entries on report forms or into a laboratory information management system, accurately calculating, recording or transcribing data as required 6.2 Ensure traceability of sample from receipt to reporting of results


Learning Outcomes



Details of Learning Activities

Teacher directed delivery of lessons

  • Pre-readings and resources
  • Laboratory techniques
  • Keeping accurate records of laboratory work
  • Simulated LIMS (laboratory information management system)
  • Interactive activities to explore theory concepts and apply knowledge


Teaching Schedule

Week

Date

Knowledge Evidence

Laboratory Practical Skills

Assessments

1

12 Feb

TOPIC 1
Biological systems, classification & taxonomy

Classifying organisms
Apply classification keys to
identify organisms

AT1 Knowledge Evidence
AT2 Lab Records
AT3 Perform Skills

2

19 Feb

TOPIC 2

Organic molecules 1 Proteins

PRAC 1 Lab orientation
Legal, ethics & safety (WHS)

AT3 Procedure 1 Classification

Skills assessment

Classification

3

26 Feb

Organic molecules 2  
Carbohydrates, lipids

PRAC 2 Lab technique #1
Accurate pipetting and mixing

 

4

4 Mar

TOPIC 3

Controls & reference material

PRAC 3 Lab technique #2
Quality control & L-J charts

AT1 2 Legal, ethics, WHS (Prac 1)

5

11 Mar

MONDAY Public Holiday
No theory class

PRAC 4 Lab technique #3
Spectrophotometry

 

6

18 Mar

TOPIC 4
Cells 1

Structures & function

PRAC 5 Multistep procedure
Putting it together:
Protein assay

AT1 3 Controls (Topic 3 Qs)

7

25 Mar

Cells 2
Plant vs animal

PRAC 6 Skills assessment Procedure 2 Protein assay Spectrophotometry

AT3 Procedure 2 Protein assay

AT2 Pracs 3-6 (Lab Records-1)

29 Mar - 7 April           Mid-semester break [includes Easter] – No lab or theory            

8

8 Apr

Logbook & LIMS grading | feedback | AT1 KE Qs

Catch up
Only for students who need it

 

9

15 Apr

Cells 3
Membrane transport

PRAC 7 Electrophoresis #1
Prepare agarose gel

 

10

22 Apr
Pub Hol 25

Cells 4
Biologically important ions

THURSDAY Public Holiday
No prac class

AT1 4 Cells (Topic 4 Qs)

11

29 Apr

TOPIC 5
Genetics 1
Nucleic acids & DNA

PRAC 8 Electrophoresis #2
Separate DNA, visualise, interpret

AT3 Procedure 3


AT1 5 Organic molecules
(Topics 2 & 4 Qs)

12

6 May

Genetics 2
Chromosomes & karyotypes

PRAC 9 Skills assessment Procedure 3 Electrophoresis

AT2 Pracs 7-9 (Lab Records-2)

13

13 May

Genetics 3
Cell cycle, mitosis, meiosis

Catch up
Only for students who need it

AT3 Procedure 3

14

20 May

Genetics 4

Inheritance patterns

PRAC 10 Skills assessment
Procedure 3
Multistep staining - Histology

AT1 6 Genetics (Topic 5 Qs)

AT2 Prac 10 (Lab Records-3)

15

27 May

Forward thinking – where the course will lead you

PRAC 11 EIA immunoassay

Multichannel pipettes & serial doubling dilutions

AT1AT2
Final corrections

16

3 Jun

Last Chance – Finalise AT Required students only

 

 


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Assessment may include quizzes, observation of laboratory skills, logbook & reports


Assessment Tasks

AT1 Knowledge Evidence
Sets of questions for each topic area that cover the theoretical concepts
Submit as indicated in Canvas

AT2
Lab Records
Logbook documentation of samples, work planning, results, calculations & interpretation
Submit as indicated in Canvas

AT3
Perform Skills
Classify organisms using dichotomous keys
Three observed multistep procedures: spectrophotometry, DNA electrophoresis, histological staining 
Assessed ondates indicated in Canvas


Assessment Matrix

Other Information

To complete the course successfully, you need to achieve a satisfactory result for every assessment.

For each assessment you may have two attempts to demonstrate the required skills.
The teacher may only offer a third attempt after discussion with the Program Coordinator. It is not automatic.

Assessment Matrix

The assessment matrix demonstrates how assessment tasks align with the relevant unit of competency. These mapping documents are available through Program Administration if required.

Other Information

This course is graded in accordance with competency-based assessment

This course is an ungraded competency-based unit of study.
The final outcome may be: 

CA Competency Achieved  
NYC Not Yet Competent
DNS Did Not Submit for assessment

Individual assessment tasks are marked Satisfactory | Not Satisfactory | Not Observed

To pass the course you need to achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence. 

Attendance

If you are sick you should submit a medical certificate to the program coordinator and course coordinator/teacher.
If you miss an assessable practical class you need to provide a medical certificate (or other evidence).

If you miss a practical class that is a prac report write-up you need to arrange an alternative report with your teacher.

Submission of Assessment Tasks

You are expected to prepare all assignments and other written work in a professional manner. More information on general academic study and writing skills can be found on the “Learning Lab” http://emedia.rmit.edu.au/learninglab/

Submit or perform all assessment tasks by their due dates.

You must keep a copy of all submitted assessments including electronic copies.

 

Academic Integrity and Plagiarism including AI  

RMIT University has a strict policy on plagiarism and academic integrity. Please refer to the website for more information on this policy. 
https://www.rmit.edu.au/students/student-essentials/assessment-and-exams/academic-integrity  

Use of Artificial Intelligence (AI) has certain restrictions in this course. Please refer to assessment instructions for guidelines on the conditions for the use of AI tools in assessments.

 

Extensions of Time for Submission of Assessable Work

If you are prevented from submitting an assessment on time by circumstances outside your control you may apply for an extension of up to seven (7) calendar days by using the Extension tool in Canvas at least one working day before the due date. The Program Coordinator, not your teacher, will decide whether to grant the extension, and inform you if the application is approved.

If you need an extension of greater than seven days, you must apply for special consideration.

Special Consideration

If you are unable to attend on the due date of an assessment (eg test, practical assessment), because of illness or other unforeseen circumstance, you may submit an APPLICATION FOR SPECIAL CONSIDERATION within 5 working days of the due date. If Special Consideration is granted, you will be given the opportunity to complete/repeat the assessment task at a later date. For advice on this application please visit www.rmit.edu.au/students/specialconsideration or speak to your Program Coordinator.

Course Overview: Access Course Overview