Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term1 2014

Course Code: ONPS5655C

Course Title: Perform aseptic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

there are no pre-requisites or co-requisites for this course


Course Description

This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973004A Perform aseptic techniques


1. Prepare for aseptic sampling or transfer

Performance Criteria:

1.1 Ensure any sampling procedure conforms tothe requirements of the sampling plan
1.2. Use specified personal protective clothing and equipment
1.3. Prepare the work area for safe and effective sample transfer
1.4. Select equipment and materials specified by the procedure
1.5. Organise equipment to minimise contamination during manipulations
1.6. Label containers for clear identification
1.7. Record details in relevant log or database


2. Transfer materials aseptically

Performance Criteria:

2.1. Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel
2.2. Sterilise inoculating loops and/or pipette where used to prevent contamination
2.3. Perform transfer while minimising opportunities for contamination and cross-infection
2.4. After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility
2.5. Re-sterilise inoculating loops, minimising the generation of aerosols
2.6. Perform quality control checks, if required
2.7. Label transport or culture vessels for clear identification



3. Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:

3.1 Maintain organised work area to prevent cross contamination

Learning Outcomes

Details of Learning Activities

Lab classes, - using aseptic techniques

short talks

Lab manual questions

Teaching Schedule

week beginning  
7.4.14IntroductionHandwashing prac

Plating out

Make media




 Quality Control

Disinfectant comparison

Gram stain

5.5 14

Biohazard cabinet

Oil immersion review

Plateout in biohazard cabinet

Gram stain- oil immersion


Plate out in biohazard cabinet

Antibiotic sensitivity set up

19.5.14Glossary review

Senstiivity reading

Plate reading

Learning Resources

Prescribed Texts


Other Resources

Library and internet

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

Final Practical assessment   26/5/14   Competent or NYC

Completed lab manual with answers to questions ongoing during sessions (C/NYC)

On line quizzes

One practical report due 8.5.14 on prac completed week of 28/4

Assessment Matrix

Other Information

Assessment Information

• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac reports).

• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.

• Late work that is submitted without an application for an extension will not be corrected

• If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.

Course Overview: Access Course Overview