Course Title: Apply routine chromatographic techniques

Part B: Course Detail

Teaching Period: Term2 2018

Course Code: BIOL5233C

Course Title: Apply routine chromatographic techniques

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5363 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Namrita Kaul

Course Contact Phone: +61 3 9925 4837

Course Contact Email: namrita.kaul@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Paul Burgio
paul.burgio@rmit.edu.au

Kirsten Balding
kirsten.balding@rmit.edu.au

 

Nominal Hours: 120

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

Pre-requisites:

 

MSL974006 Perform biological procedures

OR         

MSL974003 Perform chemical tests and procedures

Course Description

This unit of competency covers the ability to analyse samples using routine chromatographic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising workplace procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975009 Apply routine chromatographic techniques

Element:

1 Establish client needs and schedule analysis

Performance Criteria:

1.1 Liaise with client or sample provider to determine client needs and sample history 1.2 Record sample description, compare with specification and record and report any discrepancies 1.3 Identify non-routine samples and the possible need to vary workplace procedures 1.4 Seek advice from supervisor about any proposed variations and document all approved changes 1.5 Schedule analysis using workplace procedures

Element:

2 Prepare samples and standards

Performance Criteria:

2.1 Obtain a representative analytical portion of the laboratory sample 2.2 Prepare sample in accordance with testing requirements 2.3 Prepare validation checks for analytical portion

Element:

3 Set up and optimise instrument

Performance Criteria:

3.1 Perform pre-use and safety checks in accordance with workplace procedures 3.2 Start up and condition the instrument using workplace procedures 3.3 Optimise instrumental parameters to suit sample and test requirements 3.4 Check calibration status of instrument and perform calibration using specified standards and procedures, as required

Element:

4 Perform analysis

Performance Criteria:

4.1 Measure analyte response for standards, validation checks and samples 4.2 Conduct sufficient measurements to obtain reliable data 4.3 Return instruments to standby or shutdown condition, as required

Element:

5 Process and analyse data

Performance Criteria:

5.1 Confirm data is the result of valid measurements 5.2 Perform required calculations and ensure results are consistent with standards or estimations and expectations 5.3 Record results with the appropriate accuracy, precision and units 5.4 Analyse trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel 5.5 Troubleshoot analytical procedure or equipment problems which have led to atypical data or results

Element:

6 Maintain a safe work environment

Performance Criteria:

6.1 Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method 6.2 Use personal protective equipment (PPE) and safety procedures specified for test method and materials to be tested 6.3 Minimise the generation of waste and environmental impacts 6.4 Ensure the safe disposal of laboratory waste 6.5 Clean, care for and store equipment and consumables in accordance with workplace procedures

Element:

7 Maintain laboratory records

Performance Criteria:

7.1 Enter approved data and results into laboratory information management system (LIMS) 7.2 Maintain equipment logs in accordance with workplace procedures 7.3 Maintain security and confidentiality of laboratory data and workplace information 7.4 Communicate results to appropriate personnel


Learning Outcomes



Details of Learning Activities

practical classes

Online theory 

group work

worksheets

online activities

 


Teaching Schedule

 Week   DateActivity Assessment 
 1 2/7 Practical 1:
1.Course Overview
2.Safety in the Lab
3. Making solutions

 Theory (lecture online)
Paper Chromatography Theory - concepts & calcs

 
 2 9/7 Practical 2:
Paper Chromatography of food dyes - (Method optimisation & effect of the solvent)

 Theory (lecture online)
Paper Chromatography Theory

 
 3 16/7 Practical 3:
Interpreting results
& Complete Group Report in class

 Theory (lecture online)
TLC Theory concepts & calcs

1. PRACTICAL REPORT 1 (Paper Chromatography- Optimisation of the solvent)  

2. THEORY ONLINE QUIZ 1 TLC, Paper chroma

423/7 Practical 4: 
Thin Layer Chromatography of Vegetable pigments
Needs Rf values for validity
(Method optimisation & effect of the solvent)

 

Theory (lecture online)
Size Exclusion Chromatography (SEC) Theory (also called gel filtration chromatography (GFC)

 
 5 30/7 Practical 5: 
1. Solution Making for HPLC
2. SEC extraction of GFP and/or BFP from E. coli cells.

 Theory (lecture online)
Ion Exchange (IE) Chromatography Theory

 THEORY ONLINE QUIZ 2 SEC, IEC
 6 6/8 Practical 6:
SDS-PAGE of  GFP and BFP fractions

 Theory (lecture online)
Affinity chromatography (AC)
Multistep chromatography – concepts

 
 7 13/8 Revsion LOGBOOK & LAB SKILLS CHECKLIST REVIEW
 8 20/8 MID-SEMESTER THEORY EXAM
  27/8 Student break
93/9 Practical 7: 
Record results of GFP SDS-PAGE
Ion Exchange Chromatography

 Theory (lecture online)
HPLC theory & calculations - peak area, retention time, internal standards
Solid Phase Extraction (SPE)

THEORY ONLINE QUIZ 3 AC, HIC, SPE
1010/9 Practical 8:
HPLC of caffeine in drinks
(whole class)
VALID METHOD

Case studies – Common problems and Troubleshooting

 Theory (lecture online)
HPLC - isocratic v's gradient chromatography theory

 
1117/9 Practical 9:
HPLC of caffeine in drinks - interpreting results

 Theory (lecture online)
Caffeine in Drinks - Using standards to find the concentration of a substance

PRACTICAL REPORT 2 (HPLC of Caffeine in drinks spreadsheet assignment) 

 

1224/9 Practical 10:
1a. Prepare Tablets for HPLC
1b. HPLC of Tablets
VALID METHOD

2.  TLC of aspirin, caffeine & paracetamol 
VALID METHOD

 Theory  (lecture online)
Qualitative and Quantitative HPLC – Using standards to find what substances are in a sample and at what concentration

THEORY ONLINE QUIZ 4 HPLC
131/10 Practical 11:
HPLC of analgesics- interpreting results

 Theory  (lecture online)
HPLC Analgesics results
Internal standards

 
148/10 Practical 12:
GC @ Applied Chemistry Gas Chromatography of Xylene

 
Theory  (lecture online)
GC theory & calculations

LOGBOOK & LAB SKILLS CHECKLIST Final Review

 THEORY ONLINE QUIZ 5 GC Experiment

1515/10 Revision 
1622/10 END OF SEMESTER THEORY EXAM


Learning Resources

Prescribed Texts


References


Other Resources

Recommended: Boyer R (2006)  Biochemistry Laboratory Modern Theory and Techniques, Benjamin Cummings Call No. 572.078 B791 

The text has good information about both Electrophoresis and Chromatography Techniques. There are a number of copies on the library that you can borrow.

Download your practical manual from Canvas and PRINT a copy.

 


Overview of Assessment

online quizzes

practical reports

written tests

laboratory logbook


Assessment Tasks

 Assessment Task Due Date Value
 Practical Report- Paper Chromatography 20/7 15%
 Online quizzes weeks 3, 5, 9, 12 20%
 Mid semester theory exam week 8 25%
 Prac report- HPLC 17/9 15%
 Log book & lab skills 8/10 S/NYS
 End of semester exam week 16 25%

 


Assessment Matrix

Other Information

Assessment Information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment

CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

 

  • To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component)
  • A minimum of 80% attendance at practical classes is required to pass this course. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher
  • Late work that is submitted without an application for an extension (see below) will not be corrected.
  • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application should be emailed to the Program Coordinator (namrita.kaul@rmit.edu.au). Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
  • For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration

 

Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher. 

 

Course Overview: Access Course Overview