Course Title: Perform biological procedures

Part B: Course Detail

Teaching Period: Term2 2020

Course Code: BIOL5246C

Course Title: Perform biological procedures

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5388 - Diploma of Laboratory Technology

Course Contact: Amber Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Nominal Hours: 130

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

None

Course Description

This unit of competency describes the skills and knowledge to interpret work requirements, prepare samples, and perform routine biological procedures. These procedures may involve several steps and are used to classify organisms and biologically active compounds by analysing their biological and/or chemical characteristics. This unit includes interpretation of results and troubleshooting obvious departures from standard procedures.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974021 Perform biological procedures

Element:

1. Interpret and schedule work requirements

Performance Criteria:

1.1 Review work request to identify samples,required procedures and materials/equipment/instruments involved

1.2 Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment

1.3 Plan parallel work sequences to optimise throughput of multiple sets of samples

Element:

2. Receive and prepare biological samples

Performance Criteria:

2.1 Log samples using standard operating procedures (SOPs)

2.2 Record sample descriptions, compare with specification and note and report discrepancies

2.3 Prepare samples in accordance with testing requirements

Element:

3. Perform techniques that assist in the classification of organisms

Performance Criteria:

3.1 Identify characteristics used to classify organisms

3.2 Use dichotomous keys to identify organisms

Element:

4. Perform biological procedures

Performance Criteria:

4.1 Set up and use equipment and reagents in accordance with the method

4.2 Perform techniques in accordance with the standard operating procedures

Element:

5. Maintain a safe work environment

Performance Criteria:

5.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

5.2 Minimise the generation of waste

5.3 Safely dispose of biohazardous waste

5.4 Clean, care for and store equipment and reagents as required

Element:

6. Maintain laboratory records

Performance Criteria:

6.1 Record entries on report forms or into a laboratory information management system, accurately calculating, recording or transcribing data as required

6.2 Ensure traceability of sample from receipt to reporting of results


Learning Outcomes



Details of Learning Activities

Teacher directed deliver of lessons

  • Class discussion
  • Case study analysis
  • Laboratory experiments
  • Record keeping of experiments
  • Research activities
  • Site visits


Teaching Schedule

week

Date 2020

Theory

Practical/"Field trip"

Assessment

1

6-Jul

Topic 1 – Classification, taxonomy and biological systems

Field trip preparation
Dichotomous key activity

 

2

13-Jul

Topic 2 – Animal and plant cells Simulated Field trip to Carlton gardens to complete Dich Key

AT2 – Make Dichotomous key classification (Birds)

AT2 – Dichotomous key classification

3

20-Jul

Topic 3 - Animal and plant tissue types, Plant and animal cell modeling

AT2 – Use Dichotomous key (Trees)

AT2 – Dichotomous key classification

4

27-Jul

Topic 4 – Organic molecules 1 (Characteristic of organic molecules & Proteins)

Introduction - Biuret Online - Sample - calculate results; Sample traceablity & Records

AT3 - Labskills & Logbook
Due in Lab class for face to face grading and feedback

5

3-Aug

Topic 5 – Biochem lab and controls

Assessment - Biuret Online - Sample traceablity & Records, Sample - calculate results; - Intro L&J control charts

AT3 - Labskills & Logbook
Due in Lab class for face to face grading and feedback

6

10-Aug

Topic 6 – Organic molecules 2 (Carbs and fats)

Feedback - Biuret Online L&J control charts

AT3 - Labskills & Logbook
Due in Lab class for face to face grading and feedback

7

17-Aug

Quiz 1 - Organic molecules 1 & 2, animal and plant tissue types

Intro to Dye electrophoresis

AT1-Part 1 Knowledge Evidence Quiz

8

24-Aug

Log book grading and feedback

Dichotomous keys Catch up

AT3 - Labskills & Logbook
Due in Lab class for face to face grading and feedback

 

31-Aug

Student vacation

   

9

7-Sep

Topic 7 – Cell membrane and transport

Rapid Spectrophotometric Protein Determination Biuret Method (1)

L4 – Record results and answer questions

10

14-Sep

Topic 8 – Cell cycle, mitosis & meiosis

Rapid Spectrophotometric Protein Determination Biuret Method (2)

L5 – Record results and answer questions

11

21-Sep

Topic 9 - Organic molecules 3 (Chromosomes and nucleic acids)

PRAC Rapid Spectrophotometric Protein Determination Biuret Method

 

12

28-Sep

Log book grading and feedback

Catch up: Biuret Spectrophotometry

L6 – Image of nucleic acid model

13

5-Oct

Topic 10 – Organic molecules 4 (biologically significant ions)

Dye Electrophoresis (1/2 class)
(prepoured gels - students just load)

L7 – Record results and answer questions

14

12-Oct

Topic 11 – Genetics Tutorial 4

Dye Electrophoresis (1/2 class)
(prepoured gels - students just load)

L8 - Record results and answer questions for Prac 5
OR L9 - Record results and answer questions for Tutorial 4

15

19-Oct

AT1: Quiz 2 – Genetics, biologically significant ions

Catch up: Dye Electrophoresis

AT1-Part 2 Knowledge Evidence Quiz

16

26-Oct

AT2: Log book grading and feedback

AT3 - Labskills & Logbook - Dye Electrophoresis

AT2b Logbook (L4 – L9)
Due in class for face to face grading and feedback

 


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Assessment may include

reports

logbook

folio of completed tasks

observation of laboratory skills


Assessment Tasks

AT1-Part 1: Knowledge evidence quiz 1

AT1-Part 2: Knowledge evidence quiz 2

AT2-Dichotomous key classification

AT3-Labskills and logbook


Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessments

CA Competency Achieved

NYC Not Yet Competent

DNS Did Not Submit for assessment

 

To pass course students need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence

Late work that is submitted without an application for an extension (see below) will not be corrected

 

APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student my apply for an extension up to 7 days from the original due date. They must lodge the application form (HTTP://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application should be emailed to the program coordinator (kirsten.balding@rmit.edu.au). Students requiring longer extensions must apply for SPECIAL CONSIDERATION.

 

*For missed assessments such as tests- the student (& their doctor if sick) must fill out a special consideration form. this form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment http://www1.rmit.edu.au/students/specialconsideration

 

Plagiarism is the presentation of work, idea or creation of another person as though it is your own. It is a form of cheating and it is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or copy your own work.Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

 

 

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