Course Title: Perform biological procedures
Part B: Course Detail
Teaching Period: Term2 2021
Course Code: BIOL5246C
Course Title: Perform biological procedures
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5388 - Diploma of Laboratory Technology
Course Contact: Rauha Quazi
Course Contact Phone: +61 3 9925 4277
Course Contact Email: rauha.quazi@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Nominal Hours: 130
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
None
Course Description
This unit of competency describes the skills and knowledge to interpret work requirements, prepare samples, and perform routine biological procedures. These procedures may involve several steps and are used to classify organisms and biologically active compounds by analysing their biological and/or chemical characteristics. This unit includes interpretation of results and troubleshooting obvious departures from standard procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974021 Perform biological procedures |
Element: |
1. Interpret and schedule work requirements |
Performance Criteria: |
1.1 Review work request to identify samples,required procedures and materials/equipment/instruments involved 1.2 Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment 1.3 Plan parallel work sequences to optimise throughput of multiple sets of samples |
Element: |
2. Receive and prepare biological samples |
Performance Criteria: |
2.1 Log samples using standard operating procedures (SOPs) 2.2 Record sample descriptions, compare with specification and note and report discrepancies 2.3 Prepare samples in accordance with testing requirements |
Element: |
3. Perform techniques that assist in the classification of organisms |
Performance Criteria: |
3.1 Identify characteristics used to classify organisms 3.2 Use dichotomous keys to identify organisms |
Element: |
4. Perform biological procedures |
Performance Criteria: |
4.1 Set up and use equipment and reagents in accordance with the method 4.2 Perform techniques in accordance with the standard operating procedures |
Element: |
5. Maintain a safe work environment |
Performance Criteria: |
5.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel 5.2 Minimise the generation of waste 5.3 Safely dispose of biohazardous waste 5.4 Clean, care for and store equipment and reagents as required |
Element: |
6. Maintain laboratory records |
Performance Criteria: |
6.1 Record entries on report forms or into a laboratory information management system, accurately calculating, recording or transcribing data as required 6.2 Ensure traceability of sample from receipt to reporting of results |
Learning Outcomes
Details of Learning Activities
Teacher directed deliver of lessons
- Class discussion
- Case study analysis
- Laboratory experiments
- Record keeping of experiments
- Research activities
- Site visits
Teaching Schedule
BIOL5246C Perform Biological Procedures - Class schedule
Semester 2, 2021
Theory: All students are required to attend theory classes every week.
These classes will help you complete the Knowledge Evidence Quizzes.
Labs: Please print out a hardcopy of the lab manual and logbook in time for the week 4 class.
Groups: L21A, L21B, L21C: L21A: Wednesday 3-6pm, L21B: Thursday 11.30-2.30, L21C: Thursday 8.30-11.30
Each group will be split into half and each lab run twice:
You may only attend your allocated class, as numbers are strictly limited due to COVID.
Split class groups are: L21A-1, L21A-2; L21B-1, L21B-2; L21C-1, L21C-2
Week |
Date 2021 |
Theory |
Practical |
Group |
Assessment – to update |
1 |
12 Jul |
Topic 1 – Classification, taxonomy and biological systems |
Lab 1 Safety induction: Make Dichotomous key classification (Birds) |
L21A L21B L21C |
|
2 |
19 Jul |
Topic 2 – Animal and plant cells |
Lab 2 Use/Make Dichotomous keys |
L21A-1 L21B-1 L21C-1 |
AT2 – Dichotomous key classification |
3 |
26 Jul |
Topic 3 - Animal and plant tissue types, Plant and animal cell modelling |
Lab 2 Use Dichotomous keys |
L21A-2 L21B-2 L21C-2 |
AT2 – Dichotomous key classification |
4 |
2 Aug |
Topic 4 – Organic molecules 1 (Characteristic of organic molecules & Proteins) |
Lab 3 Biuret Introduction - Calculate results; Sample traceability & Records – Create a L&J control chart Rapid Biuret Protein assay #1 |
L21A-1 L21B-1 L21C-1 |
AT3 - Practical Observations; Labskills, Logbook & LIMS* |
5 |
9 Aug |
Topic 5 – Biochem lab and controls, standards (also called calibrators), Legal, ethical and WHS in the Biochem lab |
Lab 3 Biuret Introduction - Calculate results; Sample traceability & Records Rapid Biuret Protein assay #1 |
L21A-2 L21B-2 L21C-2 |
AT3 - Practical Observations; Labskills, Logbook & LIMS* |
6 |
16 Aug |
Topic 6 – Organic molecules 2 (Carbohydrates and Fats) |
Lab 4 Rapid Biuret Protein assay #2 L-J control charts Sign off Logbooks & LIMS (Labs 3&4) |
L21A-1 L21B-1 L21C-1 |
AT3 - Practical Observations; Labskills, Logbook & LIMS* |
7 |
23 Aug |
Quiz 1 - Organic molecules 1 & 2, Animal and Plant tissue types |
Lab 4 Rapid Biuret Protein assay #2 L-J control chart Sign off Logbooks & LIMS (Labs 3&4) |
L21A-2 L21B-2 L21C-2 |
AT1-Part 1 Knowledge Evidence Quiz AT3 - Practical Observations; Labskills, Logbook & LIMS* |
30 Aug |
Student vacation |
|
|
||
8 |
6 Sep |
Log book & LIMS |
Lab 5 Rapid Biuret Protein assay #3 Catch up: J control charts |
L21A-1 L21B-1 L21C-1 |
AT3 - Practical Observations; Labskills, Logbook & LIMS* |
9 |
13 Sep |
Topic 7 – Cell membrane and transport |
Lab 5 Rapid Biuret Protein assay #3 Catch up: J control charts |
L21A-2 L21B-2 L21C-2 |
AT3 - Practical Observations; Labskills, Logbook & LIMS* |
10 |
20 Sep |
Topic 8 – Cell cycle, mitosis & meiosis |
Catch ups: Rapid Biuret Protein assay #4 (for students who don’t have 2 good results yet) Dichotomous keys (s/c only) |
Required students only |
AT3 - Practical Observations; Labskills, Logbook & LIMS* |
11 |
27 Sep |
Topic 9 - Organic molecules 3 (Chromosomes and nucleic acids) |
Lab 6 Dye electrophoresis 1a Make gel & load samples |
L21A-1 L21B-1 L21C-1 |
AT3 - Practical Observations: Labskills & Logbook* |
12 |
4 Oct |
Log book & LIMS |
Lab 6 Dye electrophoresis 1b Make gel & load samples |
L21A-2 L21B-2 L21C-2 |
AT3 - Practical Observations: Labskills & Logbook* |
13 |
11 Oct |
Topic 10 – Organic molecules 4 (biologically significant ions) |
Lab 7 DNA Electrophoresis 1a |
L21A-1 L21B-1 L21C-1 |
AT3 - Practical Observations: Labskills & Logbook* |
14 |
18 Oct |
Topic 11 – Genetics Tutorial 4 |
Lab 7 DNA Electrophoresis 1b |
L21A-2 L21B-2 L21C-2 |
AT3 - Practical Observations: Labskills & Logbook* |
15 |
25 Oct |
AT1: Quiz 2 – Genetics, biologically significant ions |
Catch ups: Rapid Biuret Protein assay #5 (for students who don’t have 2 good results yet), Finalise Labskills & Logbook |
Required students only
|
SUBMIT SIGNED OFF & COMPLETED LOGBOOK TO CANVAS AT1-Part 2 Knowledge Evidence Quiz |
16 |
1 Nov |
Last Chance: Finalise Quizzes Required students only |
Last Chance: Finalise Logbook & LIMS Required students only |
Required students only
|
SUBMIT SIGNED OFF & COMPLETED LOGBOOK TO CANVAS |
*Subject to change.
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessment may include
reports
logbook
folio of completed tasks
observation of laboratory skills
Assessment Tasks
AT1-Part 1: Knowledge evidence quiz 1
AT1-Part 2: Knowledge evidence quiz 2
AT2-Dichotomous key classification
AT3-Labskills and logbook
Assessment Matrix
Other Information
This course is graded in accordance with competency-based assessments
CA Competency Achieved
NYC Not Yet Competent
DNS Did Not Submit for assessment
To pass course students need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence.
Late work that is submitted without an application for an extension (see below) will not be corrected.
APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student my apply for an extension up to 7 days from the original due date. They must lodge the application form (HTTP://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application should be emailed to the program coordinator (kirsten.balding@rmit.edu.au). Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
*For missed assessments such as tests- the student (& their doctor if sick) must fill out a special consideration form. this form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment http://www1.rmit.edu.au/students/specialconsideration
Plagiarism is the presentation of work, idea or creation of another person as though it is your own. It is a form of cheating and it is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or copy your own work.Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
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