Course Title: Perform biological procedures
Part B: Course Detail
Teaching Period: Term1 2022
Course Code: BIOL5246C
Course Title: Perform biological procedures
School: 535T Social Care and Health
Campus: City Campus
Program: C5388 - Diploma of Laboratory Technology
Course Contact: Rauha Quazi
Course Contact Phone: +61 3 9925 4277
Course Contact Email: rauha.quazi@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Amber Mitton
Nominal Hours: 130
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
None
Course Description
This unit of competency describes the skills and knowledge to interpret work requirements, prepare samples, and perform routine biological procedures. These procedures may involve several steps and are used to classify organisms and biologically active compounds by analysing their biological and/or chemical characteristics. This unit includes interpretation of results and troubleshooting obvious departures from standard procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974021 Perform biological procedures |
Element: |
1. Interpret and schedule work requirements |
Performance Criteria: |
1.1 Review work request to identify samples, required procedures and materials/equipment/instruments involved1.2 Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and/or equipment 1.3 Plan parallel work sequences to optimise throughput of multiple sets of samples |
Element: |
2. Receive and prepare biological samples |
Performance Criteria: |
2.1 Log samples using standard operating procedures (SOPs) 2.2 Record sample description, compare with specification and note and report discrepancies 2.3 Prepare samples in accordance with testing requirements |
Element: |
3. Perform techniques that assist in the classification of organisms |
Performance Criteria: |
3.1 Identify characteristics used to classify organisms 3.2 Use dichotomous keys to identify organisms |
Element: |
4. Perform biological procedures |
Performance Criteria: |
4.1 Set up and use equipment and reagents in accordance with the method 4.2 Perform techniques in accordance with the standard operating procedures |
Element: |
5. Maintain a safe work environment |
Performance Criteria: |
5.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel 5.2 Minimise the generation of waste 5.3 Safely dispose of biohazardous wastes 5.4 Clean, care for and store equipment and reagents as required |
Element: |
6. Maintain laboratory records |
Performance Criteria: |
6.1 Record entries on report forms or into a laboratory information management system, accurately calculating, recording or transcribing data as required 6.2 Ensure traceability of sample from receipt to reporting of results |
Learning Outcomes
Details of Learning Activities
Teacher directed deliver of lessons
- Class discussion
- Case study analysis
- Laboratory experiments
- Record keeping of experiments
- Research activities
- Site visits
Teaching Schedule
Theory: All students are required to attend theory classes every week.
Monday 10:30 – 12:30
These classes will help you complete the Knowledge Evidence Questions. We will be signing of this assessment task during classes.
Theory classes are linked to Assessment task 1 (AT1) – Knowledge assessment task
Part 1 (Topics 1 - 4) is due each week for signoff, and then submit completed and signed off task to Canvas at Week 5
Part 2 (Topic 6) is due each week for signoff, and then submit completed and signed off task to Canvas at end of Week 7
Part 3 (Topics 7 & 8) is due each week for signoff, and then submit completed and signed off task to Canvas at end of Week 9
Part 4 (Topics 9 & 10) is due each week for signoff, and then submit completed and signed off task to Canvas at end of Week 11
Part 5 (Topic 11) is due each week for signoff, and then submit completed and signed off task to Canvas at end of Week 15
Labs: Each lab will run twice: A practice and an assessment. Attendance to all lab classes is compulsory.
Week |
Date 2021 |
Theory |
Practical |
Assessment – to update |
1 |
7 Feb |
Topic 1 – Classification, taxonomy and biological systems |
Lab 1 Safety induction: Make Dichotomous key classification (Birds) |
|
2 |
14 Feb |
Topic 2 – Animal and plant cells |
Lab 2 Practice Use/MakeDichotomous keys |
|
3 |
21 Feb |
Topic 3 - Animal and plant tissue types, Plant and animal cell modelling |
Lab 2 Assessment UseDichotomous keys |
AT2 – Dichotomous key classification |
4 |
28 Feb |
Topic 4 – Organic molecules 1 (Characteristic of organic molecules & Proteins) |
Lab 3 Practice Biuret Introduction - Calculate results; Sample traceability & Records – Create a L&J control chart Rapid Biuret Protein assay #1 |
|
5 |
7 Mar |
Topic 5 – Biochem lab and controls, standards (also called calibrators), Legal, ethical and WHS in the Biochem lab |
Lab 3 Assessment Biuret Introduction - Calculate results, Sample traceability & Records Rapid Biuret Protein assay #1 |
AT3 - Practical Observations; Lab skills, Logbook & LIMS* AT1 – Part 1 to be uploaded to Canvas |
6 |
14 Mar |
|
Lab 4 Practice Rapid Biuret Protein assay #2 L-J control charts |
|
7 |
21 Mar |
Log book & LIMS (and AT1 questions) |
Lab 4 Assessment Rapid Biuret Protein assay #3 L-J control chart Sign off Logbooks & LIMS (Labs 3&4) |
AT3 - Practical Observations; Lab skills, Logbook & LIMS* AT1 – Part 2 to be uploaded to Canvas |
8 |
28 Mar |
Log book & LIMS (and AT1 questions) |
Lab 5 Practice Rapid Biuret Protein assay #4 Catch up: L-J control charts |
|
9 |
4 Apr |
Topic 7 – Cell membrane and transport |
Lab 5 Assessment Rapid Biuret Protein assay #5 |
AT3 - Practical Observations; Labskills, Logbook & LIMS* AT1 – Part 3 to be uploaded to Canvas |
10a |
11 Apr |
Topic 8 – Cell cycle, mitosis & meiosis |
Catch ups: Rapid Biuret Protein assay #6 (for students who don’t have 2 good results yet) Dichotomous keys (s/c only) |
|
10b |
18 Apr |
Student vacation – No lab or theory |
Split week 10a and 10b Student holiday from 14 – 20 April 2021 |
|
11 |
25 Apr |
Log book & LIMS (and AT1 questions) |
Catch ups: Rapid Biuret Protein assay #7 (for students who don’t have 2 good results yet) |
AT1 – Part 4 to be uploaded to Canvas |
12 |
2 May |
Topic 9 - Organic molecules 3 (Chromosomes and nucleic acids) |
Lab 6 Practice DNA electrophoresis #1 Make gel & load DNA samples |
|
13 |
9 May |
Topic 10 – Organic molecules 4 (biologically significant ions) |
Lab 6 Assessment DNA electrophoresis #2 Make gel & load DNA samples |
AT3 - Practical Observations: Labskills & Logbook* |
14 |
16 May |
Topic 11 – Genetics Tutorial 4 |
Lab 7 Practice DNA electrophoresis #3 Make gel & load DNA samples |
|
15 |
23 May |
Log book grading and feedback (and AT1 questions) |
Lab 7 Assessment DNA electrophoresis #4 Make gel & load DNA samples |
SUBMIT SIGNED OFF & COMPLETED LOGBOOK TO CANVAS (Electrophoresis) AT1 – Part 5 to be uploaded to Canvas |
16 |
30 May |
Last Chance: Finalise AT1 Questions Required students only |
Catch ups: --- Finalise Lab skills & Logbook --- Last Chance: Finalise Logbook & LIMS Required students only |
Complete Knowledge Evidence Questions SUBMIT SIGNED OFF & COMPLETED LOGBOOK TO CANVAS |
*Subject to change.
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessment may include
reports
logbook
folio of completed tasks
observation of laboratory skills
Assessment Tasks
AT1-Part 1, 2, 3, 4 and 5: Knowledge evidence quiz
AT2-Dichotomous key classification
AT3-Labskills and logbook
To successfully complete the course, you need to achieve a satisfactory result for every assessment.
You will be given two attempts for each assessment to demonstrate the required skills.
A third attempt can only be offered after discussions between teacher and the Program Coordinator and is not automatic.
Assessment Matrix
The assessment matrix demonstrates alignment of assessment tasks with the relevant unit of competency. These mapping documents are available through Program Administration if required.
Other Information
This course is graded in accordance with competency-based assessments
CA Competency Achieved
NYC Not Yet Competent
DNS Did Not Submit for assessment
To pass course students need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence.
Late work that is submitted without an application for an extension (see below) will not be corrected.
APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student my apply for an extension up to 7 days from the original due date. They must lodge the application form (HTTP://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application should be emailed to the program coordinator (kirsten.balding@rmit.edu.au). Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
*For missed assessments such as tests- the student (& their doctor if sick) must fill out a special consideration form. this form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment http://www1.rmit.edu.au/students/specialconsideration
Plagiarism is the presentation of work, idea or creation of another person as though it is your own. It is a form of cheating and it is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or copy your own work.Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
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