Course Title: Perform chemical pathology tests
Part B: Course Detail
Teaching Period: Term2 2020
Course Code: BIOL5249C
Course Title: Perform chemical pathology tests
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5388 - Diploma of Laboratory Technology
Course Contact: Kirsten Balding
Course Contact Phone: +61 3 9925 4283
Course Contact Email: kirsten.balding@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Giovanna.Mercuri@rmit.edu.au
Nominal Hours: 130
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL974021 Perform biological procedures
Course Description
This unit of competency describes the skills and knowledge to perform tests and procedures associated with the detection and monitoring of tissue and bodily fluid responses to normal physiological processes and disease through the identification and quantifying of chemical components. It covers tests and procedures that are usually associated with the discipline of clinical biochemistry.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975037 Perform chemical pathology tests |
Element: |
1. Process samples and associated request forms |
Performance Criteria: |
1.1 Identify specimens and request forms that do not comply with minimum industry requirements for labelling, identification and test requests 1.2 Record any discrepancies and indicate what action is required 1.3 Log samples, recording details that allow accurate tracking and chain of custody |
Element: |
2. Perform tests |
Performance Criteria: |
2.1 Select authorised tests indicated for the requested investigations 2.2 Conduct individual tests, or batches of tests, according to documented methodologies, applying required quality control procedures 2.3 Manage tasks and organise work to ensure efficient use of time 2.4 Flag test results that are outside accepted quality control limits 2.5 Apply quality control processes to discriminate between significant data and artefact 2.6 Confirm with supervisor any further testing requirements 2.7 Record all test data, noting any phenomena that may be relevant to the treatment of data or the interpretation of results |
Element: |
3. Maintain laboratory records |
Performance Criteria: |
3.1 Record entries on report forms or into a laboratory information management system, accurately calculating, recording or transcribing data as required 3.2 Ensure samples and associated paperwork maintain traceability throughout testing |
Learning Outcomes
Details of Learning Activities
Your primary learning mode will be online and face-to-face and learning activities will include a combination of lectures, classroom activities, analysis of simulated results and practical demonstrations.
Teaching Schedule
Schedule | ||
Session |
Week beginning |
Topic/Activity/Assessment |
SEMESTER 1 | ||
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Week 1
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06/07/2020 |
Theory: Introduction to Clinical chemistry, objectives, analytical variables, Quality Assurance Online practical: Levey Jennings QC Plots |
Week 2 |
13/07/2020 |
Theory: Automation and point of care testing (POCT) -Print automation practical manual before coming to class Practical: Automation and POCT I & II AT3 (A) (B) (D) (H)
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Week 3
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20/07/2020 |
Early feedback quiz – make sure you do your Chemical pathology quiz before 23.59 Sunday 26th July!
Theory: Iron Studies Online practical: 1. Iron Levels Spectrophotometry (Drylab Exercise) 2. Haemoglobin Electrophoresis
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Week 4
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27/07/2020 |
Theory: Liver I: Serum proteins Online practical: Protein estimation using Agarose Gel Electrophoresis
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Week 5
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03/08/2020 |
Theory: Tumour Markers I – Myeloma Online practical: Paraproteins Electrophoresis (Helena Labs) |
Week 6 |
10/08/2020 |
Theory: Toxicology Therapeutic Drug Monitoring (TDM) - Testing for Drugs Introduction to Chromatography & HPLC Online practical: HPLC: Separating analgesics using HPLC |
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Week 7
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17/08/2020 |
Theory: Liver II - Liver Function Tests (LFT’s) Online practical: Gamma glutamyl transferase (GGT) Finalise : AT1: week 1 to 7 online PRACTICAL LOG BOOK ENTRIES (A) (B) (D) (H) |
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Week 8
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24/08/2020 |
AT4: MID-SEMESTER 1 THEORY EXAM (B) and Resits |
|
31/08/2020 |
Mid-semester break |
Week 9 |
7/09/2020 |
On-site practical (part a): Group A Cholesterol Theory: Lipids I HDL/LDL Ratio (Dry lab exercise – complete calculations at home)
On-site practical (part b): Group A Thyroid stimulating hormone (TSH) ELISA Theory: ELISA immunoassays
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Week 10 |
14/09/2020 |
On-site practical (part a): Group B Cholesterol Theory: Lipids I HDL/LDL Ratio (Dry lab exercise – complete calculations at home)
On-site practical (part b): Group B Thyroid stimulating hormone (TSH) ELISA Theory: ELISA immunoassays
|
Week 11 |
21/09/2020 |
On-site practical (part a): Group A 1. Urine testing 2. Urine stix (demonstration) Theory: Renal
On-site practical (part b): Group A 1.Diffusion of glucose & starch across a dialysis membrane (demonstration) 2. Blood cells in hypertonic, hypotonic & isotonic saline (demonstration) Theory: Electrolytes & Water Balance
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Week 12 |
28/09/2020 |
On-site practical (part a): Group B 1. Urine testing 2. Urine stix (demonstration) Theory: Renal
On-site practical (part b): Group B 1.Diffusion of glucose & starch across a dialysis membrane (demonstration) 2. Blood cells in hypertonic, hypotonic & isotonic saline (demonstration) Theory: Electrolytes & Water Balance
|
Week 13 |
05/10/2020 |
On-site practical: Group A BCG Albumin limitations Beer Lambert Theory: Liver Functions I
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Week 14 |
12/10/2020 |
On-site practical: Group B BCG Albumin limitations Beer Lambert Theory: Liver Functions I
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Week 15
|
19/10/2020 |
On-site practical: Group A Total Protein First determine the Endpoint Theory: Liver Functions I
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Week 16
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26/10/2020 |
On-site practical: Group B Total Protein First determine the Endpoint Theory: Liver Functions I
Finalise : AT1: week 1 to 16 PRACTICAL LOG BOOK ENTRIES (A) (B) (D) (H) |
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Week 17
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02/11/2020 |
Exam week AT2: END OF SEMESTER 1 THEORY EXAM (B) and Re-sits Monday - Cup day (Bank holiday) |
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Week 18
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9/11/2020 |
Semester break
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Learning Resources
Prescribed Texts
References
Other Resources
Booklist
The following books are used in this course. Note that the required texts are essential for completion of the weekly questions in this course.
Useful reference for in dept study:
Pagana, Kathleen Deska, author, & Pagana, Timothy James. (2018). Mosby's manual of diagnostic and laboratory tests(Sixth ed.). St. Louis, Missouri: Elsevier Mosby. ISBN-10: 0323446639 ISBN-13: 978-0323446631
- Practical Manual: PRINT AND BRING A COPY TO CLASS. Digital copy available on Canvas.
- Automation and Point of Care Testing Manual. PRINT AND BRING A COPY TO CLASS. Digital copy available on Canvas please print and bring a copy to class – check the schedule for dates.
- LABORATORY INFORMATION SYSTEMS (LIS) DATA INPUT- On a weekly basis you will simulate a chemical pathology LIS system using the following document to record and store all patient details and valid test results throughout the semester. This document will form part of your assessment.
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Logbook: A logbook is required for this course to record all experimental details. The logbook must be brought to class each week and signed off by the teacher in addition to the observation checklist AT1.
The book needs to be a bound NON-SPIRAL exercise book.
Completion of experimental records in the logbook trains students how to keep good records that can be used as “proof of discovery”. These logbooks form legal documents that can be used to prove discovery for Intellectual Property & patenting purposes & thus form an important part of the course. SPIRAL bound books ARE NOT ACCEPTABLE.
- Required Texts
- Gaw, M. Murphy, R. Cowan, D. O'Reilly, M. Stewart, J. Shepard. (2008) Clinical Biochemistry, An illustrated colour text, 4th edition. Churchill Livingstone (Elsevier), Sydney, ISBN 9780443069321. Buy this book at the beginning of semester 1. (The 3rd ed is also ok).
- Upfal and O'Callaghan (2001) Your Medical Tests. Every person's guide to common medical tests and diagnostic images. Black Inc. Melbourne ISBN 1-86395-075-3. Chapters 6 to 11 available to download and online from RMIT library.
- Recommended Texts
This is a very nice simple text that explains lots of lab techniques, especially electrophoresis and chromatography techniques and other techniques including enzyme kinetics, spectrophotometry and molecular diagnostics.
Boyer R. (2006) Biochemistry Laboratory Modern Theory and Techniques, Benjamin Cummings - Available in the library: Call No. 572.078 B791
Overview of Assessment
Assessment may include
reports
knowledge tests
observation of laboratory skills
logbook
Assessment Tasks
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Assessment task Each task must be completed satisfactorily before Competence is achieved for the full subject/unit. |
Due Date |
*Result |
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Methods of Assessment include: |
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A. Direct Observation/ |
B. Written/Verbal |
C. Third Party |
D. Portfolio/ |
E. Project/ |
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F. Presentation |
G. Role Plays |
H. Practical Demonstration |
I. Other |
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QUIZ -EOP |
26/07/2020 |
S/NS |
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WEEK1-16 |
S/NS |
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WEEK 17* |
S/NS |
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13/07/20 |
S/NS |
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WEEK8* |
S/NS |
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Note: AT2 AND AT4 assessment dates have been swapped because term 3 and term 4 practicals have been flipped in line with a term 4 back to on-site practicals during these pandemic conditions.
Assessment Matrix
Can be found on canvas LMS system
Other Information
Decision making rules
Every task must be completed satisfactorily for competence to be achieved.
Assessment Information
CA = Competency Achieved
NYC = Not yet Competent
DNS = Did Not submit (1 or more assessments)
Late work that is submitted without an application for an extension (see below) will not be corrected.
APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher
Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
Reasonable adjustment
Reasonable adjustment is an individualised modification made to the training delivery or assessment methods to enable a student with specific needs to access and participate. Reasonable adjustment may include changed assessment conditions, flexibility in assessments, other academic support, provision of adaptive equipment and in class support.
Reasonable adjustment is applied within the start date and end date for the subject/unit being undertaken.
Course Overview: Access Course Overview