Course Title: Perform chemical pathology tests

Part B: Course Detail

Teaching Period: Term2 2021

Course Code: BIOL5249C

Course Title: Perform chemical pathology tests

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5388 - Diploma of Laboratory Technology

Course Contact: Rauha Quazi

Course Contact Phone: +61 3 9925 4277

Course Contact Email: rauha.quazi@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Rowena.Lavery@rmit.edu.au

Nominal Hours: 130

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

MSL974021  Perform biological procedures

Course Description

This unit of competency describes the skills and knowledge to perform tests and procedures associated with the detection and monitoring of tissue and bodily fluid responses to normal physiological processes and disease through the identification and quantifying of chemical components. It covers tests and procedures that are usually associated with the discipline of clinical biochemistry.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975037 Perform chemical pathology tests

Element:

1. Process samples and associated request forms

Performance Criteria:

1.1 Identify specimens and request forms that do not comply with minimum industry requirements for labelling, identification and test requests 1.2 Record any discrepancies and indicate what action is required 1.3 Log samples, recording details that allow accurate tracking and chain of custody

Element:

2. Perform tests

Performance Criteria:

2.1 Select authorised tests indicated for the requested investigations 2.2 Conduct individual tests, or batches of tests, according to documented methodologies, applying required quality control procedures 2.3 Manage tasks and organise work to ensure efficient use of time 2.4 Flag test results that are outside accepted quality control limits 2.5 Apply quality control processes to discriminate between significant data and artefact 2.6 Confirm with supervisor any further testing requirements 2.7 Record all test data, noting any phenomena that may be relevant to the treatment of data or the interpretation of results

Element:

3. Maintain laboratory records

Performance Criteria:

3.1 Record entries on report forms or into a laboratory information management system, accurately calculating, recording or transcribing data as required 3.2 Ensure samples and associated paperwork maintain traceability throughout testing


Learning Outcomes



Details of Learning Activities

This course uses Canvas LMS and is run as a combination of laboratory practical classes, workshops, and theory classes.


Teaching Schedule


Schedule

Ϯ SYLLABUS subject to changing pending pandemic conditions and RMIT & government safety advise

 

Session

Week beginning

Topic/Practical

Online/Activities/Assessment

SEMESTER 1

Week 1


12/07/2021


Theory class:

-Introduction to Clinical chemistry, Objectives, Analytical Variables, Quality Control (QC)

-QC manufacturers sheets

- Preparation for on campus practical: Lipids I, review the module and complete the quiz


Practical #1:

-Cholesterol assay

- HDL/LDL Ratio (drylab)

Week 2

19/07/2021

Theory class:

-Prep for on campus practical:Enzyme Linked Immunosorbent Assay (ELISA)

-review the modules and complete the quiz


Practical #2:

-ELISA assay - for detection of Thyroid Stimulating Hormone (TSH) simulation

 

 

Early assessment task - make sure you do your Chemical pathology quiz before 23.59 Sunday 25th July.


AT1: PRACTICAL LOG BOOK ENTRIES (A) (B) (D) (H) -submit your scanned logbooks entries weeklyonline



Week 3


26/07/2021


Theory class:

Prep for on campus practical: Renal

-review the module and complete the quiz


Practical # 3:

1. Urine testing

2. Urine stix (Multistix diagnostic urinalysis testing strips)



Week 4


02/08/2021


Theory class:

-Electrolytes -review the module and complete the quiz

-Prep for on campus practical: Liver function tests (BCG) : review the module and complete the quiz


Practical #4:

-BCG Albumin assay, limitations Beer Lambert





Week 5


9/08/2021


Theory class:

-Levey Jennings QC Plots (practice)

-Prep for on campus practical: Liver function tests (Total protein assay) : review the module and complete the quiz


Practical #5:

-Total Protein assay, first determine the Endpoint (minutes) of the reaction

 

 

 

 

Week 6

16/08/2021

 

AT2: MID-SEMESTER 1 THEORY EXAM (B)

Online theory quiz topics: Introduction to Chem pathology, QC controls & Calibrators, Reference ranges & reading results, Electrolytes, Renal, Lipids, Liver,  ELISA & Endocrinology

-knowledge quiz will be active for 3 hours starting at your usual PRACTICAL class time (on campus to be confirmed) Friday 20th August 8.30 am to 11.30 am.

Week 7


23/08/21

 

AT2: MID-SEMESTER 1 THEORY EXAM (B) – second attempt (is not automatic and at the discretion of the teacher)



30/08/2021

 

Mid-semester Break

Week 8


06/09/2021

 

Theory class:

Prep for on campus practical: Toxicology Therapeutic Drug Monitoring (TDM) - Testing for Drugs

Introduction to Chromatography & High-Performance Liquid Chromatography (HPLC)

-review the module and complete the quiz


Practical #6:

Separating analgesics using HPLC

 

Chromatograms will be available the week after this practical- submit logbook 19th September 23.59

 

 

 

Week 9

13/09/2021

 

Theory class:

Prep for on campus practical: Serum Protein Electrophoresis (SPE) using Agarose Gels

-review the module and complete the quiz

 

 Practical #7:

SPE electrophoresis (Helena Labs)

Workshop/ HelenaLab Scientist Georgina Sallmann will attend

 

Week 10

20/09/2021

 

Theory class:

Preparation for assignmentAT3 -Automation : Technological Advances

No practical lab this week due to AFL holiday - to be confirmed (TBC)

 

 

Week 11

27/09/2021

 

Theory class:

Prep for on campus practical: Iron studies

-review the module and complete the quiz

 

Practical #8:

Haemoglobin (Hb) Electrophoresis (Helena Labs)

Workshop/ HelenaLab Scientist Georgina Sallmann will attend

AT3 –AUTOMATION

complete automation quiz 1 and 2 before Sunday 26th September 23.59

Week 12

04/10/2021

 

Theory class:

Prep for on campus practical:

Liver II - Liver Function Tests (LFT’s) & Gamma Glutamyl Transferase (GGT)

-review the module and complete the quiz


Practical #9:

GGT assay

 

 

Week 13

11/10/2021

 

Theory class:

Prep for on campus practical:

Tumour Markers I – Myeloma & Paraproteins Electrophoresis (Helena Labs)

-review the module and complete the quiz

 

Practical #10:

Paraproteins Electrophoresis (Helena Labs) Workshop/ HelenaLab Scientist Georgina Sallmann will attend


Week 14

18/10/2021

AT4: END OF SEMESTER 1 THEORY EXAM (B)

Online theory quiz topics: Use of controls and calibrators, Iron studies, SPE, Tumour markers, HPLC, GGT and LFTs.

-knowledge quiz will be active for 3 hours starting at your usual PRACTICAL class time (on campus to be confirmed) Friday 22nd October 8.30 am to 11.30 am.

 

Week 15

25/10/2021

AT4: END OF SEMESTER 1 THEORY EXAM (B) second attempts (is not automatic and at the discretion of the teacher)


Week 16


1/11/2021

Semester break


Learning Resources

Prescribed Texts


References


Other Resources

Recommended Texts - useful reference for in depth study:

 

  • Pagana, Kathleen Deska, author, & Pagana, Timothy James. (2018). Mosby's manual of diagnostic and laboratory tests(Sixth ed.). St. Louis, Missouri: Elsevier Mosby. Available in the RMIT library ISBN-10: 0323446639 ISBN-13: 978-0323446631


Overview of Assessment

Assessment may include

reports

knowledge tests

observation of laboratory skills

logbook


Assessment Tasks

Assessment details

 

Assessment task

Each task must be completed satisfactorily before Competence is achieved for the full subject/unit.

Due Date

Result
S (satisfactory)
/ NS

(not satisfactory)

Methods of Assessment include:


A. Direct Observation/
    Simulation Activities

B. Written/Verbal
    Questioning

C. Third Party
     Evidence

D. Portfolio/
     Product

E. Project/
    Report


F. Presentation

G. Role Plays

H. Practical Demonstration

I. Other


QUIZ -EOP

25/07/2021

S/NS

  1. AT1- logbooks (A) (B) (D) (H)

Weekly

S/NS

  1. AT2- theory (B)

Week 6/7

S/NS

  1. AT3 – automation (A) (B)

Week 11

S/NS

  1. AT4- theory (B)

Week 14 / 15

S/NS


Assessment Matrix

Assessment mapping is available online within canvas LMS.

Other Information

Decision making rules

Every task must be completed satisfactorily for competence to be achieved.
Second attempt is not automatic and at the discretion of the teacher

 

Assessment Information

 

CA = Competency Achieved

NYC = Not yet Competent

DNS = Did Not Submit (1 or more assessments)


 

Late work that is submitted without an application for an extension (see below) will not be corrected.



APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the Program Coordinator.


For missed assessments such as exams DUE TO CIRCUMSTANCES OUT OF YOUR CONTROL- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration


Attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to the Program Coordinator and Course Coordinator. If you miss a practical class that is a practical report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher.


Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

 

Reasonable adjustment

Reasonable adjustment is an individualised modification made to the training delivery or assessment methods to enable a student with specific needs to access and participate. Reasonable adjustment may include changed assessment conditions, flexibility in assessments, other academic support, and provision of adaptive equipment and in class support.

Reasonable adjustment is applied within the start date and end date for the subject/unit being undertaken.

Course Overview: Access Course Overview