Course Title: Perform immunohaematological tests

Part B: Course Detail

Teaching Period: Term2 2021

Course Code: BIOL5251C

Course Title: Perform immunohaematological tests

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5388 - Diploma of Laboratory Technology

Course Contact: Rauha Quazi

Course Contact Phone: +61 3 9925 4277

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Andrea McAdam

Course Coordinator | Teacher

Nominal Hours: 80

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


Course Description

This unit of competency describes the skills and knowledge to perform routine tests and procedures that are part of the requirements of pre- and post-blood transfusion practice. The unit also covers tests and procedures that are indicated in laboratory investigations in obstetric and perinatal medicine, in suspected haemolysis and haemolytic episodes, and in other clinical circumstances.

This unit applies to technical officers and laboratory technicians working in the biomedical industry sector. It is understood that the management of any transfusion laboratory would establish for itself, in terms of its own responsibility and purposes, the ability of any worker to work in a transfusion science laboratory, regardless of the education and training record or presumed ability of any worker.


Tests will be related to the determination of blood groups and the detection of antibodies of significance in:

  • transfusion (as laboratory evidence that in vivo cell destruction or immunisation may occur)
  • pregnancy and the peri-natal period (as evidence of sensitisation of foetal red cells by transplacental maternal antibody)
  • the investigation of haemolysis or haemolytic episodes

The tests that the worker will use will be validated and authorised procedures, clearly described in the laboratory's manual of procedures. The unit of competency is based on the assumption that technical personnel would perform tests and procedures under the close supervision of scientific and/or medical staff. The parameters of interpretation will be clearly described, indicating for the worker what he or she is permitted to sign-off without reference to supervisors or managers.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975030 Perform immunohaematological tests


1. Process samples and associated request forms

Performance Criteria:

1.1 Identify specimens and request forms that do not comply with minimum industry requirements for labelling, identification and test requests 1.2 Record any discrepancies and indicate what action is required 1.3 Log samples, recording details that allow accurate tracking and chain of custody


2. Perform tests

Performance Criteria:

2.1 Conduct individual tests according to documented standard operating procedures, applying required quality control 2.2 Use appropriate manual techniques to determine patient/donor red cell phenotype and antibody status 2.3 Read and grade tube and card agglutination reactions 2.4 Record and interpret results 2.5 Validate test results and reagents using control cells and reagents 2.6 Complete documentation required to permit the issuing of blood or blood components that have been cleared for use by clinical staff


3. Maintain laboratory records

Performance Criteria:

3.1 Record entries on report forms or into a laboratory information management system accurately calculating, recording or transcribing data as required 3.2 Maintain records of blood and blood products received, used and returned to supplier 3.3 Ensure traceability of sample from receipt to reporting of results

Learning Outcomes

Details of Learning Activities

Students will gain hands-on experience with laboratory equipment, reagents and real blood samples (provided by Australian Red Cross Lifeblood from blood donors) in a simulated working laboratory.

Routine tests include quality assurance procedures, ABO and RhD blood grouping, screening for unexpected antibodies, Direct Antibody Test, and neonatal / maternal investigations

Features of fresh and processed blood products, and selection criteria for safe transfusion will be identified.

Experienced medical scientists will link the laboratory and theory material to industry workplace practice through demonstrating, and real-life case stories.

In-class exercises at the bench, on paper, kinaesthetic learning, online tutorials and group work will reinforce the theory.

Teaching Schedule






13 July

Equipment & reagents

Introduction to safe transfusion
What's a blood group?


20 July

Blood groups on tiles
Pre-transfusion testing guidelines

Antibodies meet antigens


27 July

Reactions & interactions

Avoiding disasters;
maintaining quality


3 Aug

Tube ABO group
Quality Control

More on red cell membranes:
are you positive?


10 Aug

Tube ABO group & QC

Transfusing blood products


17 Aug

Choosing compatible units

Laboratory testing techniques:
CAT and automation


24 Aug

CAT ABO group & QC

Other blood group systems



7 Sep

Antibody Tests

Antibody screening


14 Sep

DAT | Indirect Antibody Tests

AT2 Dry Case Studies Quiz


21 Sep

Antibody identification

Antibody Identification 


28 Sep

CAT Group & Screen x 2 
Antibody identification & QC

Phenotyping red cells


5 Oct

Phenotyping CDE K &QC

Serious Hazards of Transfusion
Analysing annual SHOT Report


12 Oct

Transfusion reactions

Antenatal screening | HDFN
Testing & transfusing neonates


19 Oct

'Mother & baby' investigation:
G&S | DAT 

Concept review and refresh


26 Oct

Practice AT6 Practical Skills 
Tube Group CAT Screen x2

AT5 Final Quiz


1-5 Nov

AT6 Practical Assessment

split smaller groups this week
this week 


8-12 Nov

Learning Resources

Prescribed Texts


Other Resources

Up-to-date links to Transfusion websites and other relevant resources are available on Canvas and within course material.

Overview of Assessment

Assessments may include

observation of laboratory skills



knowledge tests

Assessment Tasks

Assessment Task  


 Practice Ass 01

 Wk 3 27 July 2021

AT1 Pre-transfusion sample validity

 Wk 4 03 Aug

 Practice AT4 'spot audit' workbooks

 Wk 7 24 Aug

 AT2 Knowledge Test: Dry Case Studies

 Wk 8 16 Sep 

 AT3 Blood Products online

 Wk 11 03 Oct

 AT4 Work Records Portfolio submit in class

 Wk 14 19 Oct

 Practice AT6 Practical Skills

Wk 15 26 Oct

 AT5 Knowledge Test Dry Case Studies

 Wk 15 28 Oct

 AT6 Practical Skills in split groups

 Wk 16 | 17 tbc

To pass the course you need achieve a satisfactory result for every assessment.

You will be given only two attempts for each assessment to demonstrate you have the required skills, at the discretion of the teacher.

A third attempt can only be offered after discussions between the teacher and the Program Coordinator and is not automatic.

Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment

CA Competency Achieved  
NYC Not Yet Competent
DNS Did Not Submit for assessment

Submission of Assessment Tasks

Any work submitted for assessment in this course must be your own. Plagiarism is the presentation of any work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

Extensions of time for submission of assessable work

If you are prevented from submitting an assessment on time by circumstances outside your control you may apply for an extension of up to seven (7) calendar days by filling in the application form at least one working day BEFORE the due date. The application form should be emailed to your Program Coordinator, Namrita Kaul,
Eligibility criteria for application and the form is available from the link:

If you require an extension of time longer than 7 days, you must apply for Special Consideration.

Late work that is submitted without an application for an extension will not be corrected.

Special consideration

If you are unable to sit a test, exam or lab-based assessments due to unexpected circumstances (e.g., illness), you should apply for Special Consideration for the missed assessment. Information on applying for Special Consideration can be found from the link: Applications for Special Consideration should NOT be submitted to the course or program coordinator.

Course Overview: Access Course Overview