Course Title: Perform tissue and cell culture techniques
Part B: Course Detail
Teaching Period: Term2 2020
Course Code: BIOL5255C
Course Title: Perform tissue and cell culture techniques
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5388 - Diploma of Laboratory Technology
Course Contact: Kirsten Balding
Course Contact Phone: +61 3 9925 4283
Course Contact Email: kirsten.balding@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Seb Buckingham
jocesebastian.buckingham@rmit.edu.au
Nominal Hours: 100
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL973016 Perform aseptic techniques
Course Description
This unit of competency describes the skills and knowledge to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment set-up, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975033 Perform tissue and cell culture techniques |
Element: |
1.Interpret and schedule production requirements |
Performance Criteria: |
1.1 Review client request and confirm quantity and nature of cells, tissue or products |
Element: |
2. Work safely according to the legal and regulatory framework |
Performance Criteria: |
2.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and workplace quality assurance procedures |
Element: |
3. Assemble and maintain tissue culture equipment |
Performance Criteria: |
3.1 Assemble, sterilise or decontaminate equipment according to workplace procedures 3.2 Perform pre-use and safety checks in accordance with relevant workplace and operating procedures |
Element: |
4. Prepare and test cell and tissue culture media |
Performance Criteria: |
4.1 Confirm media specifications and processes/methods |
Element: |
5. Obtain, monitor and maintain tissue and cell lines |
Performance Criteria: |
5.1 Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture |
Element: |
6. Preserve cells and tissues |
Performance Criteria: |
6.1 Select the appropriate preservation method 6.2 Preserve the cell lines or tissue in accordance with the method |
Element: |
7. Maintain records |
Performance Criteria: |
7.1 Maintain records of batches of media and test data |
Learning Outcomes
Refer to elements
Details of Learning Activities
- Teacher directed delivery of lessons
- Class discussion
- Worksheets
- Laboratory experiments
- Record keeping of experiments
- Research activities
- group work
Teaching Schedule
Week | Date 2020 | Lab/Practical # | Topic | Assessment |
1 | 6-Jul | 1&2 | Orientation AND Aseptic technique in the biohazard cabinet | AT2: Logbook |
2 | 13-Jul | 3 | Pipetting and transfer of fluids | AT1 Practical Demonstration |
AT2: Logbook | ||||
3 | 20-Jul | 4 | Preparation of tissue culture solutions and sterility testing | AT1 Practical Demonstration |
AT2: Logbook | ||||
4 | 27-Jul | 5 | Morphology of INT407 cell line | AT1 Practical Demonstration |
AT2: Logbook | ||||
5 | 3-Aug | 6 | Feeding INT407 cell line, Cancel Prac 6 if necessary (this procedure is repeated in Prac 8 onwards) | AT1 Practical Demonstration |
AT2: Logbook | ||||
6 | 10-Aug | 7 | Passaging INT407 cell line using a split ratio | AT1 Practical Demonstration |
AT2: Logbook | ||||
7 | 17-Aug | Log Book (S/NYS) | AT2: Logbook | |
8 | 24-Aug | 8 | Cell counting using the manual method and automatic coulter (Group A) | AT1 Practical Demonstration |
AT2: Logbook | ||||
9 | 31-Aug | 8 | Cell counting using the manual method and automatic coulter (Group B) | AT1 Practical Demonstration |
AT2: Logbook | ||||
10 | 7-Sep | 9 | Morphology and passaging of Murine immune cells DC 2.4 (dentritic cells) | AT1 Practical Demonstration |
AT2: Logbook | ||||
11 | 14-Sep | 10 | Passaging Pancreatic Cancer PC3 cell line using a split ratio – INDIVIDUAL PRAC | AT1 Practical Demonstration |
AT2: Logbook | ||||
12 | 21-Sep | Seb away - fieldtrip - may or maynot occur | ||
13 | 28-Sep | 11&12 | Passaging a cell line using cell density AND Cell preservation, storage and recovery | AT1 Practical Demonstration |
AT2: Logbook | ||||
14 | 5-Oct | 13 | EXAM Passaging INT407 cell line using a split ratio | AT1 Practical Demonstration |
AT3: Practical Exam | ||||
15 | 12-Oct | Log Book (S/NYS) | AT2: Logbook | |
16 | 19-Oct | LogBook resubmission | AT2: Logbook | |
17 | 26-Oct | Alternate assessments | ||
(special consideration only) |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessment may include
Logbook- recording and analysis of laboratory results
Observation of laboratory tasks
online quizzes
written reports
Assessment Tasks
This course features 4 assessments conducted in class and online in Semester 1:
- Assessment Task 1: Laboratory Activities (competent/not yet competent)
- Assessment Task 2.1: Log book Prac 1 to 8 (competent/not yet competent)
- Assessment Task 2.2: Log book Prac 9 to 13 (competent/not yet competent)
- Assessment Task 3: Practical Exam (competent/not yet competent)
Assessment Matrix
Other Information
Assessment information:
This course is graded in accordance with competency-based assessment
CA Competency Achieved
NYC Not Yet Competent
DNS Did Not Submit for assessment
Submission of Assessment Tasks
It is expected that any work presented for assessment in this course will be your own. Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
Extensions of time for submission of assessable work
If you are prevented from submitting an assessment on time by circumstances outside your control you may apply for an extension of up to seven (7) calendar days by filling in the application form at least one working day BEFORE the due date. The application form should be emailed to your Program Coordinator, Kirsten Balding kirsten.balding@rmit.edu.au
Eligibility criteria for application and the form is available from the link: https://www.rmit.edu.au/students/student-essentials/assessment-and-exams/assessment/extensions-of-time-for-submission-of-assessable-work
If you require an extension of time longer than 7 days, you must apply for Special Consideration.
Late work that is submitted without an application for an extension will not be corrected.
Special consideration
If you are unable to sit a test, exam or lab-based assessments due to unexpected circumstances (e.g., illness), you should apply for Special Consideration for the missed assessment. Information on applying for Special Consideration can be found from the link: https://www.rmit.edu.au/students/student-essentials/assessment-and-exams/assessment/special-consideration. Applications for Special Consideration should NOT be submitted to the course or program coordinator.
Course Overview: Access Course Overview