Course Title: Perform microscopic examination
Part B: Course Detail
Teaching Period: Term1 2011
Course Code: ONPS5656C
Course Title: Perform microscopic examination
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Gwen MacDonald
Nominal Hours: 40
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
there are no pre-requisites or co-requisites for this course
Course Description
This unit of competency covers the ability to set up a light microscope for optimum resolution, to prepare routine samples and to observe, identify and report sample characteristics.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL973007A Perform microscopic examination |
Element: |
1. Interpret test requirements |
Performance Criteria: |
1.1 Review test request to identify samples to be tested, test method and equipment involved |
Element: |
2. Set up work area for preparation and examination of samples |
Performance Criteria: |
2.1 Collect equipment and arrange the workspace so that equipment can be used safely and efficiently |
Element: |
3. Prepare samples for examination |
Performance Criteria: |
3.1 Log and label samples according to enterprise procedures to ensure traceability |
Element: |
4. Set up and use a light microscope |
Performance Criteria: |
4.1 Set up the light path to optimise resolution |
Element: |
5. Observe, identify and report sample characteristics |
Performance Criteria: |
5.1 Recognise and identify significant sample characteristics |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1 Ensure safety and minimise cross-contamination through the use of personal protective clothing and safety equipment |
Learning Outcomes
Details of Learning Activities
Practical Classes, question sheets, tutorials
Teaching Schedule
10.2.11 | Introduction Set up procedure Ergonomic safety/OHS Microscope theory |
Microscope allocation Workbook outline |
17.2.11 | Wet preps Light characteristics Cell types |
Observation of yeasts Drawing X100,X400Llabelled broth of sacchromyces Onion slices |
24.2.11 | Parts of microscope, Resolution Blood, Stains, Storage of specimens Labelling Making blood film-cells in blood- clinical |
Blood film Wright’s stain Fixed smear observation Wet prep Slide mounting |
3.3.11 | Blood counts QC records Errors |
Auto and manual counts on patient specimen* (Prac report 20%)Due 18/3/11 |
10.3.11 | Microscope trouble shooting Tissue sections Control slides Oil introduction Measure cells |
Perl’s stain prac |
17.3.11 | Individual tutorial | Question sheet Times to be announced |
24.3.11 | Prac assessment- wet preps | Set up wet prep on labelled sample according to industry procedure |
9.5.11 | Gram stain | Gram Staining bacteria |
30.5.11 | Assessment of oil immersion | |
week of 6.6.11 | Theory test |
Learning Resources
Prescribed Texts
References
Other Resources
Access to internet, library
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
practical assessment
Assessment Tasks
Theory Test of underpinning knowledge 30%
Record of practicals done and questions in work book 50%
Practical write up 20%
Assessment of microscope set up and performance of wet preparation and oil immersion microscopy Competent/ NYC
At least 5 of 7 practicals to be completed
Assessment Matrix
Other Information
All assessment tasks must be passed to pass the subject
The 40 hours allocated for this course are
8X2 hour practical sessions 16 hours
1 report research and writing 4 hours
8 X 0.5 hour note reading and research 4 hours
Completion of workbook and other questions 2.0X8 hour
2 X 1/4 hour tutorial sessions 1 /2 hour
Practical assessments 1/2 hour
Theory assessment 1 hour
Course Overview: Access Course Overview