Course Title: Apply electrophoretic techniques
Part B: Course Detail
Teaching Period: Term1 2015
Course Code: ONPS5662C
Course Title: Apply electrophoretic techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Kirsten Balding
Kirsten.Balding@rmit.edu.au
Phone: 9925 4283 (office hours)
Nominal Hours: 100
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL973002A - Prepare working solutions OR MSL974001A - Prepare, standardise and use solutions
MSL974003A - Perform chemical tests and procedures.
Course Description
This unit of competency covers the ability to analyse samples using electrophoretic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975008A Apply electrophoretic techniques |
Element: |
1. Establish client needs and schedule analysis |
Performance Criteria: |
1.1. Liaise with client or sample provider to determine client needs and sample history |
Element: |
2. Prepare samples and standards |
Performance Criteria: |
2.1. Obtain a representative analytical portion of the laboratory sample |
Element: |
3. Set up and optimise instrument |
Performance Criteria: |
3.1. Perform pre-use and safety checks in accordance with enterprise procedures |
Element: |
4. Perform analysis |
Performance Criteria: |
4.1. Measure analyte response for standards, validation checks and samples |
Element: |
5. Process and analyse data |
Performance Criteria: |
5.1. Confirm data is the result of valid measurements |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1. Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method |
Element: |
7. Maintain laboratory records |
Performance Criteria: |
7.1. Enter approved data and results into laboratory information management system |
Learning Outcomes
Details of Learning Activities
Lab classes and Record keeping
Lectures/Workshops
Team meetings
Presentations
Exams
Reports and Assignments
Online theory and quizzes
Research
Fieldtrips
Teaching Schedule
Program is subject to change
FOR LONG EXPERIMENTS THEORY & PRAC CLASSES ARE MERGED TOGETHER
Part 1 - DNA
(week of) |
week | THEORY (at home) |
PRAC 4 Hours | Assessment |
9/2 | 1 | Intro to Electrophoresis **On campus class** |
Lab 1 Prepare Solutions Calculations No prac class this week |
Quiz (in class) Logbook |
16/2 | 2 | 1. Visualising DNA: 2. useful stains 3.. Molecular weight markers, 4. Gel concentrations 5. Traceability of results |
Lab 2 Agarose Gels I: Frankenstein Gels - Separating DNA with different % gels. Preparing solutions - Agarose gel electrophoresis (calculations) |
Quiz Logbook |
23/2 | 3 | Quantifying DNA using agarose electrophoresis and fluorimeter methods. | Lab 3 Agarose Gels II How much & how pure is my DNA? (fluorimetry versus agarose gel determination) |
Quiz Logbook |
2/3 | 4 | Which allele(s) of Alu is in your DNA? How can you tell from the electrophoresis results? |
Lab 4 Electrophorese Mol Gen Alu PCR products gel electrophoresis |
Quiz Logbook |
9/3 | 5 | Electrophoresis of RNA (Northern Blotting); Electrophoresis of Genomic DNAs (Pulsed field gel electrophoresis, Southern Blotting) | Lab 5 1. Pipetting Workshop Refresher: forward and reverse pipetting Multichannel pipettes 2. Prac report 1: in class activity: Annotate a gel photo and enter results data and analysis |
Quiz Logbook |
16/3 | 6 | Revision (at home) | ||
23/3 | 7 | Exam week | no lab | Exam 1 |
30/3 | 8a | Intro to DNA sequencing & Capillary Electrophoresis | DNA Sequencing* (AGRF Sequencing facility visit) |
Quiz |
6/4 | Student vacation | |||
13/4 | 9 | Protein Electrophoresis Types of gels: agarose, cellulose acetate, SDS-PAGE & MW calculations. | Lab 6 1. Prepare solutions for protein agarose gel & SDS- PAGE. 2. Agarose Gel Electrophoresis of Serum Proteins (SPE) (Helena Labs*) |
Quiz Logbook |
20/4 | 10 | Intro to SDS-PAGE, Calc size of proteins using SDS-PAGE |
Lab 7 Protein Agarose Electrophoresis of Serum Haemoglobins (HbE) (Helena Labs*) |
Quiz Logbook |
27/4 | 11 | SDS-PAGE | Lab 8 SDS-PAGE of Albumin and Ovalbumin (Separate proteins by size). Prac Report 2) |
Logbook Prac Report 2 - Lab component |
4/5 | 12 | SDS-PAGEprep for lab | Lab 9 1. Record results from last week 2. Prac report 2 - in class activity: Annotate a gel photo, analyse and report results. |
Quiz Logbook Prac Report 2 -analysis//results |
11/5 | 13 | SDS-PAGE prep for lab | Lab 10 SDS-PAGE of Fish Proteins. |
Quiz Logbook |
18/5 | 14 | Immunoassays |
Lab 11 Western Transfer and Blot of Fish Proteins. (BioRad*) |
Quiz (prac report due) |
25/5 | 15 | REVISION | No lab class - revision at home | |
1/6 | 16 | Exam Week | Exam 2 | Exam 2 |
8/6 | Alternative assessments (Special consideration only) | Alt Exam if required | ||
* To be confirmed |
Learning Resources
Prescribed Texts
Lab Manual - Term 1 (DNA) |
|
Resources available on Blackboard for this course |
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
- Class activities
- Reports
- Presentations
- Exams
- Online Quizzes
- Logbook records of experiments
Assessment Tasks
Assessment 1. Theory
a. Weekly Quizzes 20% (best 10 grades count @ 2% each) There is a quiz most weeks
b. 2 Theory Exams 25% + 25% = 50% Week of 23/3/15 and Week of 1/6/15
Assessment 2. Prac Reports
1: Agarose Electrophoresis of DNA 15%
Lab: 26/2 or 5/3/15 (choose week 3 or 4)
Analysis/writeup: 12/3/15 (week 5)
2. 2: SDS-PAGE of Albumin 15%
Lab: 30/4/15 (Week 11)
Analysis/writeup: 7/5/15 (week 12)
Assessment 3. Logbook and Lab Skills Record
Satisfactory
(satisfactory records kept for at least 80% of lab classes)
Use the self assessment checklist to see if you have completed the record, then get it assessed within 2 weeks of the class. To be considered satisfactory each record will be assessed against the checklist and signed off by the supervisor on the central logbook checklist sheet.
There will be a mid-semester review of logbook and lab skills followed by a final assessment
Total 100%
Assessment Matrix
Other Information
Time Allowance for this course
The 100 nominal hours for this unit are allocated as follows:
Theory at home = 17hrs
10 online weekly quizzes = 15hrs
revision at home = 10hrs
exams 1& 2 = 4hrs
lab = 43hrs
fieldtrip = 4hrs
report write ups = 7hrs
Total Hours = 100
This course is graded in accordance with competency-based assessment, but which also utilises graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (60 – 79%)
CC Competent with Credit (50 – 59%)
CAG Competency Achieved – Graded (0 – 49%)
NYC Not Yet Competent
DNS Did Not Submit for assessment
Assessment information
- All assessment types must be passed (exams, prac, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac component).
- Attendance at practical classes is compulsory. A minimum of 80% attendance is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator
- Late work that is submitted without an application for an extension will not be corrected.
- APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51, or emailed to the Coordinator (amber.mitton@rmit.edu.au).
- Students requiring extensions longer than 7 days must apply for Special Consideration (see the ‘Help me’ link in blackboard, via myRMIT studies or http://www1.rmit.edu.au/students/specialconsideration) For missed assessments such as exams - you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence (e.g. medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
- If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.If you miss a prac class you must provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.
Course Overview: Access Course Overview